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AAT Bioquest

FAQ

Posted on June 6, 2023
What are the differences between peptide and protein?
Bioconjugation Chemical ReagentsDye to Peptide ConjugationPeptide and OligonucleotidePhysiological ProbesProteinamino acid sequence


Posted on June 6, 2023
What are the similarities between peptide and protein?
Bioconjugation Chemical ReagentsDye to Peptide ConjugationPeptide and OligonucleotidePhysiological ProbesProteinamino acid sequence


Posted on June 6, 2023
What are the functions of peptides?
Bioconjugation Chemical ReagentsDye to Peptide ConjugationPeptide and OligonucleotidePhysiological ProbesProteinamino acid sequence


Posted on March 21, 2023
What is the chemical mechanism by which the peptide bond is formed?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence


Posted on March 21, 2023
What do peptide bonds hold together?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence


Posted on March 21, 2023
What are the different forms of peptide bonds?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence


Posted on March 21, 2023
How is peptide bond broken?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence


Posted on March 21, 2023
Why is peptide bond planar?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence


Posted on March 21, 2023
What are the chemical properties of peptides?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence


Posted on March 21, 2023
What are the physical properties of peptides?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence


Posted on March 21, 2023
What catalyzes the formation of peptide bonds?
Antibodies and ProteomicsEnzymesPeptide and OligonucleotideProteinamino acid sequence


Posted on March 21, 2023
What are the benefits of having peptide bonds?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence


Posted on March 21, 2023
What are the characteristics of peptide bonds?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence


Posted on March 1, 2023
What are the common primers used in reverse transcription?
DNA and RNA QuantitationDNA and RNAPCR primerPeptide and OligonucleotidePolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)


Posted on November 14, 2020
How do you purify oligonucleotide conjugates by HPLC?
Bioconjugation Chemical Reagents Peptide and Oligonucleotide LabelingConjugationPeptide and Oligonucleotide


Posted on November 3, 2020
How do I determine cytotoxicity?
Peptide and OligonucleotideChemical ReagentsFörster Resonance Energy Transfer (FRET)


Posted on October 19, 2020
Why does my DNA oligonucleotide have mutations?
Peptide and Oligonucleotide LabelingDNADNA and RNAPeptide and Oligonucleotide


Posted on October 19, 2020
How long can I store the oligonucleotide?
Peptide and Oligonucleotide LabelingPeptide and OligonucleotideStorage


Posted on July 22, 2020
What methods are commonly used to label the probes in fluorescence in situ hybridization (FISH)?
DNA and RNAEnzymesFISHFluorescencePeptide and OligonucleotidePolymerase Chain Reaction (PCR)in situ hybridization


Posted on July 22, 2020
What is nick translation?
DNADNA and RNADNA sequencingFISHPeptide and Oligonucleotidein situ hybridization


Posted on July 22, 2020
How do antisense oligonucleotides work in gene silencing?
DNADNA and RNANucleic AcidsPeptide and OligonucleotideRNAgene sequencing


Posted on June 22, 2020
What types of protein tags are commonly used?
Peptide and OligonucleotideProteinProtein PurificationPurification


Posted on June 22, 2020
What are the 3 binding sites for tRNA in the ribosome?
Cellular processesDNADNA and RNANucleic AcidsPeptide and OligonucleotideProteinRNACell Structure and Organelles


Posted on June 22, 2020
What are the three stages of translation?
Cellular processesDNADNA and RNANucleic AcidsPeptide and OligonucleotideProteinRNA


Posted on June 22, 2020
What is a codon?
DNADNA and RNANucleic AcidsPeptide and OligonucleotideProteinRNA


Posted on June 22, 2020
What is protein denaturation?
Antibodies and ProteomicsPeptide and OligonucleotideProtein


Posted on June 22, 2020
What are the 4 levels of protein structure?
Antibodies and ProteomicsPeptide and OligonucleotideProtein


Posted on June 22, 2020
What are the main interactions that stabilize each level of protein structure?
Antibodies and ProteomicsPeptide and OligonucleotideProtein


Posted on June 22, 2020
What is the relationship between protein structure and protein function?
Antibodies and ProteomicsPeptide and OligonucleotideProtein


Posted on June 22, 2020
What are the main protein types and their functions?
Antibodies and ProteomicsEnzymesPeptide and OligonucleotideProtein


Posted on June 22, 2020
What level of protein structure is not disturbed by denaturing?
Antibodies and ProteomicsPeptide and OligonucleotideProtein


Posted on June 22, 2020
How do acids and bases denature a protein?
Antibodies and ProteomicsPeptide and OligonucleotideProtein


Posted on June 22, 2020
What is the difference between protein degradation and denaturation?
Antibodies and ProteomicsPeptide and OligonucleotideProtein


Posted on June 22, 2020
What are the differences between the detection methods based on a fluorescent probe and an intercalating dye in qPCR?
DNADNA and RNA QuantitationPCRPeptide and OligonucleotidePolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on May 9, 2020
What are reverse transcription primers?
Antibodies and Proteomics Peptide and Oligonucleotide LabelingCellular ProcessesDNA and RNADNA and RNA QuantitationPeptide and OligonucleotideRNAPCR primer


Posted on May 9, 2020
What are oligonucleotides?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNAFRETPeptide and Oligonucleotidefluorescence imaging


Posted on April 24, 2020
Is there a difference between tagging GFP at the N-terminus and at the C-terminus of a gene?
Peptide and Oligonucleotide LabelingPeptide and OligonucleotideProtein


Posted on April 24, 2020
What is the difference between recombinant and synthetic polypeptides?
Antibodies and Proteomics BioconjugationDNA and RNAPeptide and OligonucleotideProtein


Posted on April 24, 2020
Which method is better for measuring the amount of antibodies in a sample, ELISA or FACS?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)ELISAEnzymesPeptide and Oligonucleotide


Posted on April 24, 2020
What is the difference between 'operon' and 'transcription unit'?
Cellular ProcessesCellular Structures and OrganellesDNADNA and RNAEnzymesNucleic AcidsPeptide and OligonucleotideProteinRNA